# Hydrolysis rates from bulk samples, plate reader results: EN556 # C. Arnosti (UNC) # version: 2017-11-16 # ========================= cruise_id station cast depth_no depth_m substrate rep1_rate rep2_rate rep3_rate average std_dev ------------------------- EN556 1 1 d1 1 a-glu 1.13 0.93 2.54 1.53 0.88 EN556 1 1 d1 1 b-glu 0.17 0 0 0.06 0.1 EN556 1 1 d1 1 L 0 0 0.01 0 0 EN556 1 1 d1 1 AAF 0 0 0.01 0 0 EN556 1 1 d1 1 AAPF 0 0 0 0 0 EN556 1 1 d1 1 QAR 1.62 1.45 0.8 1.29 0.43 EN556 1 1 d1 1 FSR 0 0 0 0 0 EN556 1 1 d2 58 a-glu 0.29 0.2 0.03 0.17 0.13 EN556 1 1 d2 58 b-glu 0.33 0.23 0.21 0.26 0.06 EN556 1 1 d2 58 L 0 0 0 0 0 EN556 1 1 d2 58 AAF 4.7 4.23 4.4 4.44 0.24 EN556 1 1 d2 58 AAPF 0 0 0 0 0 EN556 1 1 d2 58 QAR 2.8 1.8 1.46 2.02 0.7 EN556 1 1 d2 58 FSR 0 0 0 0 0 EN556 2 2 d1 1 a-glu 0.05 0.02 0 0.02 0.02 EN556 2 2 d1 1 b-glu 0.09 0.16 0.23 0.16 0.07 EN556 2 2 d1 1 L 0 0.01 0 0 0 EN556 2 2 d1 1 AAF 3.73 2.55 1.51 2.6 1.11 EN556 2 2 d1 1 AAPF 0 0 0 0 0 EN556 2 2 d1 1 QAR 1.53 0.6 0.75 0.96 0.5 EN556 2 2 d1 1 FSR 0 0 0 0 0 EN556 2 2 d2 78 a-glu 0.07 0.12 0.03 0.07 0.05 EN556 2 2 d2 78 b-glu 0.17 0 0 0.06 0.1 EN556 2 2 d2 78 L 0 0 0 0 0 EN556 2 2 d2 78 AAF 2.48 2.72 2.28 2.49 0.22 EN556 2 2 d2 78 AAPF 0 0 0 0 0 EN556 2 2 d2 78 QAR 2.41 0 0.39 0.93 1.3 EN556 2 2 d2 78 FSR 0 0 0 0 0 EN556 3 3 d1 1 a-glu 0.37 0.45 0.72 0.52 0.18 EN556 3 3 d1 1 b-glu 4.06 2.5 1.2 2.58 1.43 EN556 3 3 d1 1 L 82.4 72.58 60.93 71.97 10.75 EN556 3 3 d1 1 AAF 21.13 14.47 2.65 12.75 9.36 EN556 3 3 d1 1 AAPF 37.36 20.33 8.11 21.93 14.69 EN556 3 3 d1 1 QAR 6.59 5.04 0.28 3.97 3.29 EN556 3 3 d1 1 FSR 5.02 15.1 2.39 7.5 6.71 EN556 3 3 d2 97 a-glu 1.28 0.56 0.35 0.73 0.49 EN556 3 3 d2 97 b-glu 0.65 0.83 0.82 0.77 0.1 EN556 3 3 d2 97 L 26.94 16.6 15.88 19.81 6.19 EN556 3 3 d2 97 AAF 23 0.36 5.62 9.66 11.85 EN556 3 3 d2 97 AAPF 11.34 1.69 1.02 4.68 5.77 EN556 3 3 d2 97 QAR 66.58 1.46 3.19 23.74 37.11 EN556 3 3 d2 97 FSR 3.78 7.62 0 3.8 3.81 EN556 4 4 d1 1 a-glu 0.52 0 0 0.17 0.3 EN556 4 4 d1 1 b-glu 1.61 1.14 1.48 1.41 0.24 EN556 4 4 d1 1 L 92.74 88.86 58.39 80 18.81 EN556 4 4 d1 1 AAF 4.13 4.82 1.14 3.37 1.96 EN556 4 4 d1 1 AAPF 18.05 11.11 5.37 11.51 6.35 EN556 4 4 d1 1 QAR 15.96 28.18 9.75 17.97 9.38 EN556 4 4 d1 1 FSR 14.81 13.39 14.68 14.29 0.78 EN556 4 4 d2 199 a-glu 0 0 0 0 0 EN556 4 4 d2 199 b-glu 4.33 0.85 0.13 1.77 2.25 EN556 4 4 d2 199 L 32.47 18.02 0.97 17.16 15.77 EN556 4 4 d2 199 AAF 0 3.15 0 1.05 1.82 EN556 4 4 d2 199 AAPF 18.42 19.49 12.93 16.95 3.52 EN556 4 4 d2 199 QAR 7.43 3.66 3.26 4.79 2.3 EN556 4 4 d2 199 FSR 7.47 0 0 2.49 4.31 EN556 5 13 d1 2 a-glu 0.22 0.21 0 0.15 0.13 EN556 5 13 d1 2 b-glu 1.24 1.81 0.63 1.23 0.59 EN556 5 13 d1 2 L 24.74 22.53 22.72 23.33 1.22 EN556 5 13 d1 2 AAF 0 0 0 0 0 EN556 5 13 d1 2 AAPF 16.05 29.84 85.03 43.64 36.5 EN556 5 13 d1 2 QAR 22.83 24.94 27.6 25.12 2.39 EN556 5 13 d1 2 FSR 4.83 10.48 5.08 6.8 3.19 EN556 5 13 d2 35 a-glu 1.04 1.76 0.51 1.1 0.63 EN556 5 13 d2 35 b-glu 1.59 1.5 0.88 1.32 0.38 EN556 5 13 d2 35 L 24.95 15.63 11.82 17.47 6.76 EN556 5 13 d2 35 AAF 8.75 0 0 2.92 5.05 EN556 5 13 d2 35 AAPF 24.84 14.94 11.68 17.15 6.85 EN556 5 13 d2 35 QAR 3.21 3.25 0 2.15 1.86 EN556 5 13 d2 35 FSR 5.92 5.83 0 3.92 3.39 EN556 5 13 d3 330 a-glu 0 0 0 0 0 EN556 5 13 d3 330 b-glu 0 0 0 0 0 EN556 5 13 d3 330 L 0 0 0 0 0 EN556 5 13 d3 330 AAF 0 0 0 0 0 EN556 5 13 d3 330 AAPF 0 0 0 0 0 EN556 5 13 d3 330 QAR 0 0 0 0 0 EN556 5 13 d3 330 FSR 0 0 1.94 0.65 1.12 EN556 5 12 d4 750 a-glu 0.53 0.3 0.34 0.39 0.12 EN556 5 12 d4 750 b-glu 0.3 0.54 0.02 0.29 0.26 EN556 5 12 d4 750 L 14.54 3.37 0 5.97 7.61 EN556 5 12 d4 750 AAF 2.74 0 0 0.91 1.58 EN556 5 12 d4 750 AAPF 13.77 18.04 9.61 13.81 4.21 EN556 5 12 d4 750 QAR 0 0 0 0 0 EN556 5 12 d4 750 FSR 3.26 0 11.1 4.79 5.71 EN556 5 12 d5 1974 a-glu 0 0 0 0 0 EN556 5 12 d5 1974 b-glu 0.6 0.32 0.02 0.32 0.29 EN556 5 12 d5 1974 L 10.98 11.02 0.89 7.63 5.83 EN556 5 12 d5 1974 AAF 1.45 0.5 0 0.65 0.74 EN556 5 12 d5 1974 AAPF 5.66 2.75 3.75 4.05 1.48 EN556 5 12 d5 1974 QAR 0 0 0 0 0 EN556 5 12 d5 1974 FSR 4.17 5.81 0 3.33 3 EN556 6 11 d1 2 a-glu 2.23 0 0.73 0.99 1.14 EN556 6 11 d1 2 b-glu 4.72 5.42 2.94 4.36 1.28 EN556 6 11 d1 2 L 29.61 31.59 26.73 29.31 2.44 EN556 6 11 d1 2 AAF 1.75 0 0 0.58 1.01 EN556 6 11 d1 2 AAPF 127.93 22.77 61.01 70.57 53.23 EN556 6 11 d1 2 QAR 24 21.69 20.43 22.04 1.81 EN556 6 11 d1 2 FSR 8.47 0.42 0 2.96 4.77 EN556 6 11 d2 35 a-glu 0.6 0.57 0.4 0.52 0.11 EN556 6 11 d2 35 b-glu 2.82 4.55 4.14 3.84 0.91 EN556 6 11 d2 35 L 45.67 19.28 34.92 33.29 13.27 EN556 6 11 d2 35 AAF 18.32 12.67 27.23 19.41 7.34 EN556 6 11 d2 35 AAPF 36.25 34.92 46.07 39.08 6.09 EN556 6 11 d2 35 QAR 25.22 18.87 16.27 20.12 4.61 EN556 6 11 d2 35 FSR 13 15.45 11.54 13.33 1.98 EN556 6 10 d3 300 a-glu 1.13 1.27 0.77 1.06 0.26 EN556 6 10 d3 300 b-glu 0 2.21 1.04 1.08 1.11 EN556 6 10 d3 300 L 15.18 19.29 6.28 13.58 6.65 EN556 6 10 d3 300 AAF 9.02 27.97 3.55 13.51 12.82 EN556 6 10 d3 300 AAPF 4.58 12.76 6.56 7.97 4.26 EN556 6 10 d3 300 QAR 3.92 14.82 2.14 6.96 6.86 EN556 6 10 d3 300 FSR 0.47 15.24 0 5.24 8.67 EN556 6 10 d4 2800 a-glu 0 0.79 0 0.26 0.46 EN556 6 10 d4 2800 b-glu 0.47 0 0 0.16 0.27 EN556 6 10 d4 2800 L 4.56 3.94 0.65 3.05 2.1 EN556 6 10 d4 2800 AAF 0 0 0 0 0 EN556 6 10 d4 2800 AAPF 0.66 0 0 0.22 0.38 EN556 6 10 d4 2800 QAR 9.03 2.75 1.5 4.42 4.03 EN556 6 10 d4 2800 FSR 6.21 12.27 10.47 9.65 3.11 EN556 6 10 d5 3500 a-glu 0 0 0 0 0 EN556 6 10 d5 3500 b-glu 0 0.41 0.41 0.27 0.24 EN556 6 10 d5 3500 L 19.09 11.78 12.46 14.44 4.04 EN556 6 10 d5 3500 AAF 0 0 8.92 2.97 5.15 EN556 6 10 d5 3500 AAPF 9.01 0 0 3 5.2 EN556 6 10 d5 3500 QAR 13.45 4.47 10.01 9.31 4.53 EN556 6 10 d5 3500 FSR 5.32 4.01 2.01 3.78 1.67 EN556 7 9 d1 2 a-glu 1.07 0 0 0.36 0.62 EN556 7 9 d1 2 b-glu 22.14 23.21 11.6 18.98 6.41 EN556 7 9 d1 2 L 38.58 36.56 36.09 37.08 1.32 EN556 7 9 d1 2 AAF 15.16 23.8 5.25 14.73 9.28 EN556 7 9 d1 2 AAPF 24.13 23.75 16.82 21.57 4.12 EN556 7 9 d1 2 QAR 4.63 1.59 9.43 5.21 3.96 EN556 7 9 d1 2 FSR 18.3 58.66 19.21 32.06 23.05 EN556 7 9 d2 30 a-glu 0 0.2 0 0.07 0.11 EN556 7 9 d2 30 b-glu 7.16 7.47 4.28 6.3 1.76 EN556 7 9 d2 30 L 55.39 53.86 54.48 54.58 0.77 EN556 7 9 d2 30 AAF 18.78 12.68 12.55 14.67 3.55 EN556 7 9 d2 30 AAPF 20.76 47.54 23.34 30.55 14.78 EN556 7 9 d2 30 QAR 23.56 15 28.54 22.37 6.85 EN556 7 9 d2 30 FSR 9.43 16.26 7.54 11.08 4.58 EN556 7 8 d3 285 a-glu 0 0 0 0 0 EN556 7 8 d3 285 b-glu 0.46 0.23 0.06 0.25 0.2 EN556 7 8 d3 285 L 23.77 21.94 22.6 22.77 0.93 EN556 7 8 d3 285 AAF 19.78 19.47 25.9 21.72 3.63 EN556 7 8 d3 285 AAPF 5.12 3.97 3.46 4.18 0.85 EN556 7 8 d3 285 QAR 0.39 0 0 0.13 0.22 EN556 7 8 d3 285 FSR 0 3.65 0 1.22 2.11 EN556 7 8 d4 2800 a-glu 1.41 0 0 0.47 0.82 EN556 7 8 d4 2800 b-glu 1.13 1.33 2.81 1.75 0.92 EN556 7 8 d4 2800 L 4.37 4.5 1.55 3.47 1.67 EN556 7 8 d4 2800 AAF 0 0 0 0 0 EN556 7 8 d4 2800 AAPF 6.28 0 0 2.09 3.63 EN556 7 8 d4 2800 QAR 0.5 0 0 0.17 0.29 EN556 7 8 d4 2800 FSR 9.36 11.3 12.41 11.02 1.54 EN556 7 8 d5 4080 a-glu 0.08 0 0 0.03 0.04 EN556 7 8 d5 4080 b-glu 1.1 1.27 0.57 0.98 0.37 EN556 7 8 d5 4080 L 14.95 0 7.44 7.46 7.47 EN556 7 8 d5 4080 AAF 1.74 0 16.18 5.97 8.88 EN556 7 8 d5 4080 AAPF 7.55 0 0 2.52 4.36 EN556 7 8 d5 4080 QAR 0.08 0 2.25 0.78 1.28 EN556 7 8 d5 4080 FSR 0.5 8.01 0 2.84 4.49 EN556 8 7 d1 5 a-glu 0.15 0 0.26 0.14 0.13 EN556 8 7 d1 5 b-glu 2.26 1.71 1.11 1.69 0.57 EN556 8 7 d1 5 L 25.79 16.05 27.57 23.14 6.2 EN556 8 7 d1 5 AAF 0 0 0 0 0 EN556 8 7 d1 5 AAPF 14.79 12.78 7.02 11.53 4.03 EN556 8 7 d1 5 QAR 19 9.51 20.06 16.19 5.81 EN556 8 7 d1 5 FSR 4.2 5.38 0.49 3.36 2.55 EN556 8 6 d2 24 a-glu 0.46 0.64 0.17 0.42 0.23 EN556 8 6 d2 24 b-glu 1.05 1.71 0.8 1.19 0.47 EN556 8 6 d2 24 L 7.17 17.08 20.99 15.08 7.12 EN556 8 6 d2 24 AAF 3.88 8.99 0 4.29 4.51 EN556 8 6 d2 24 AAPF 16.89 18.95 4.22 13.36 7.97 EN556 8 6 d2 24 QAR 9.11 5.35 5.7 6.72 2.08 EN556 8 6 d2 24 FSR 4.25 6.47 1.56 4.09 2.46 EN556 8 6 d3 300 a-glu 0 0 0 0 0 EN556 8 6 d3 300 b-glu 0.1 0 0 0.03 0.06 EN556 8 6 d3 300 L 2.94 0 0 0.98 1.7 EN556 8 6 d3 300 AAF 5.35 15.89 1.62 7.62 7.4 EN556 8 6 d3 300 AAPF 17.32 14.2 7.97 13.16 4.76 EN556 8 6 d3 300 QAR 15.07 2.96 0 6.01 7.98 EN556 8 6 d3 300 FSR 6.25 3.21 2.69 4.05 1.92 EN556 8 6 d4 2800 a-glu 0 0 0 0 0 EN556 8 6 d4 2800 b-glu 2.24 0 0.27 0.84 1.22 EN556 8 6 d4 2800 L 15.07 2.74 0 5.94 8.03 EN556 8 6 d4 2800 AAF 2.56 0 0 0.85 1.48 EN556 8 6 d4 2800 AAPF 7.59 0 0 2.53 4.38 EN556 8 6 d4 2800 QAR 12.02 7.37 0 6.47 6.06 EN556 8 6 d4 2800 FSR 3.4 1.89 0.48 1.93 1.46 EN556 8 5 d5 4574 a-glu 0.13 0 0 0.04 0.08 EN556 8 5 d5 4574 b-glu 0 0 0 0 0 EN556 8 5 d5 4574 L 8.28 0 0 2.76 4.78 EN556 8 5 d5 4574 AAF 0 0 0 0 0 EN556 8 5 d5 4574 AAPF 5.66 0 0 1.89 3.27 EN556 8 5 d5 4574 QAR 8.95 0.49 0 3.15 5.03 EN556 8 5 d5 4574 FSR 5.76 NA 5.91 5.84 0.1